Comparative evaluation of RBPT and CFT for detection of Brucella antibodies from ruminants

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  • October 22, 2021
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Azizah Darus. Comparative evaluation of RBPT and CFT for detection of Brucella antibodies from ruminants. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of e-Poster Presentation).


Azizah Darus.  Veterinary Research Institute, Department of Veterinary Services, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia.  Corresponding author: 

Brucellosis is an economically important zoonotic disease which is endemic in many countries. The screening is based mainly on serological testing including Complement Fixation Test (CFT) and Rose Bengal Plate Test (RBPT).  CFT is time-consuming and technically a complex test. Meanwhile, RBPT is a faster, simple plate agglutination test with low non-specific reactions. Previously, the Malaysian Department of Veterinary Services (DVS) uses the in-house CFT as confirmation of Brucellosis.  Currently, DVS is using the commercial RBPT as a screening test before confirmation by CFT. In this study, 336 sera of ruminants from various farms throughout Malaysia were evaluated. From 336 sera, 245(72.9%) and 214(63.7%) were found to be positive  by the RBPT and CFT, respectively.  205(61.0%) serum samples were found positive by both RBPT and the CFT.  CFT detected Brucella antibodies in 9 (2.7%) serum samples which were negative by the RBPT. 40 (11.9%) serum samples which were found positive by the RBPT were found negative by CFT. By comparing the overall results, RBPT detects more positive samples than CFT. The present study showed a higher sensitivity but the lower specificity of the RBPT compared to CFT. Successful control programmes should be implemented based on the use of simple tests of high sensitivity, such as the RBPT then followed by confirmatory tests of high specificities, such as CFT. Precaution should be taken for animals found negative by the RBPT but positive to CFT.  

Keywords: Brucellosis, serologic screening, Complement Fixation Test (CFT), Rose Bengal Plate Test (RBPT)    

Categories: 2018
Tags: Jurnal/Prosiding/Abstrak