Comparison of bacteria culture and polymerase chain reaction (PCR) methods in identification of Corynebacterium pseudotuberculosis in clinical samples from goat
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- October 22, 2021
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Nur Anis Adilah Binti Rosli. Comparison of bacteria culture and polymerase chain reaction (PCR) methods in identification of Corynebacterium pseudotuberculosis in clinical samples from goat. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of e-Poster Presentation).
1Nur Anis Adilah Binti Rosli, 2Siti Nor Binti Che Yahya1Veterinary Research Institute, Department of Veterinary Services,59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia;2UNIVERSITI MALAYSIA KELANTAN, Karung Berkunci 36, Taman Bendahara,16100 Pengkalan Chepa, KelantanCorresponding author: firstname.lastname@example.org
Corynebacterium pseudotuberculosis is responsible to cause Caseous Lymphadenitis (CLA) commonly in sheep and goat. Therefore, it causes major economic loss in small ruminant industry. Early detection is vital for the control and prevention steps of this disease. Currently, identification of C. pseudotuberculosis mainly depends on microbiological examination, followed by biochemicalidentification. They are fastidious organisms, growing slowly even on enriched medium. Hence to fasten the detection, polymerase chain reaction (PCR) technique was developed by targeting the geneof these bacteria which is phospholipase D (pld). In this study, a total of 7 pus samples from 7 goats were obtained from the nine semi-intensive farms involving the total population of 289 goats collectively. All the goats were clinically examined for the present of the abscessation on the external lymph nodes. The samples collected were further diagnosed with two techniques which are bacterial culture and PCR. Isolation via bacteria culture was carried out by using the blood agar made out of 5% defibrinated sheep blood, subsequently sub-culture into the Brain Heart Infusion agar (BHI) and biochemical test was conducted. The PCR method also been conducted to all the samples by using the specific primer for pld gene (band=203 bp). The study reveals that 6/7 (85.71%) of the pus sample were positive with C. pseudotuberculosis by bacterial culture while 7/7 (100%) of the pus sample were positive by the PCR. Hence, PCR can significantly improve rapid and sensitive C. pseudotuberculosis detection and could supersede bacteriological culture for microbiological and epidemiological diagnosis of CLA.