CONSTRUCTION OF KNOCK-OUT FUR GENE FOR THE PRODUCTION OF IROMPs IN PASTERURELLA MULTOCIDA 6: B FROM CATTLE

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  • October 22, 2021
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RAMLAN, M., NURUL AZYLA, A., SITI NAHDATUL ISNAINI, S.H., NORLINDAWATI, A.P & BOHARI, M.J.
Veterinary Research Institute, 59 Jalan Sultan Azlan Shah, 31400 Ipoh, Perak

Haemorrhagic Septicaemia (HS) is a major disease of cattle and buffaloes characterized by an acute, highly fatal septicaemia with high morbidity and mortality. In Asia, HS caused by infection with Pasteurella multocida serotype B:2 or 6:B. To overcome the problem associated with the use of currently available vaccine the quality and effectiveness of vaccine need to be improved. The production of IROMPs is regulated by Fur protein by repressing the expression of the respective genes when complexed with iron. Fur (ferric uptake regulator) protein is a DNA-binding protein which regulates iron-responsive gene. The inactivation or deletion of Fur gene avoids the repression effect of the transcription of the downstream sequence of the genome which eventually leads to uncontrollable production of IROMPs. Therefore, it is predicted that all IROMPs will be produced in fur-knockout strain when grown in BHI and has a good potential to be used to prepare a subunit haemorrhagic septicamia vaccine based on IROMPs.

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