Detection of Avian Reovirus in commercial broiler-breeder chicken in Johor, Malaysia by Reverse Transcriptase Polymerase Chain Reaction

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  • October 22, 2021
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Mohammad Fhitri Shari. Detection of Avian Reovirus in commercial broiler-breeder chicken in Johor. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of e-Poster Presentation).

 

Mohammad Fhitri Shari.  Veterinary Research Institute, Department of Veterinary Services, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia.  Corresponding author: alongfhitri@gmail.com 

Avian reoviruses (ARVs) are members of the Orthoreovirus genus in the family Reoviridae. These viruses were recognized to be associated with many avian diseases such as tenosynovitis, respiratory and enteric diseases, hydropericardium, pericarditis, myocarditis and hepatitis which lead to significant economic loss due to loss of production, poor feed conversion, increased culling and carcass condemnations. The objective of this study which was conducted on April 2018 was to screen the presence of ARV’s antigen in commercial broiler-breeder chicken flocks in Johor, Malaysia via Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). The study population has been reported to show clinical signs such as lameness and stunting syndrome since 15 weeks old, in April 2017. The samples such as tendon, ceca tonsils and pooled organs (lung, liver, spleen, heart and kidney) were collected at four different processing plants to represent the overall population of the farm. A total of 40 tendons, 40 ceca tonsils and 40 sets of pooled organs samples were collected. All the samples were subjected to RT-PCR for ARV’s antigen detection. Result shows that ARV was detected 70% (30/40), 50% (20/40) and 0% (0/40) from the tendons, ceca tonsils and pooled organs samples respectively. The resistance and pathogenicity of the virus probably be the reasons for the presence of the virus in tendon and ceca samples. In conclusion, vaccination programme against ARV infection in the study population is recommended to control the disease.  

Keywords: Avian reovirus (ARV), Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), broiler-breeder chicken    

Categories: 2018
Tags: Jurnal/Prosiding/Abstrak
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