Isolation and detection of Equine Influenza Virus in nasal swabs from outbreak cases in Malaysia

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  • October 22, 2021
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Roslina Hassan. Isolation and detection of Equine Influenza Virus in nasal swabs from outbreak cases in Malaysia. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of e-Poster Presentation).

 

Roslina Hassan, Nurshuhada Abd Hamid, Zuraidah Othman, Siti Suraya Hani Mohd Salim, Roshaslinda Dahlan, Norlina Daiyan, Hasrul Abu Hassan, Zunaida Barker  

Veterinary Research Institute, Department of Veterinary Services, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia  

Corresponding author: rlina0876@yahoo.com 

Equine influenza virus (EIV) belongs to Influenza A group and causes respiratory syndrome disease in horses, leading to economic loses. The disease has a worldwide occurrence and is easily transmitted by direct contact with infected animals, fomites and aerosols. This study was proposed to utilize Specific Pathogen Free (SPF) eggs with the assistance of one-step reverse transcriptase (RT-PCR) assay to facilitate EIV isolation and diagnosis from field samples. A total of 547 nasal swabs from horses were received by Veterinary Research Institute from August to December 2015 with respiratory disease symptoms. Out of 547 samples tested, four were positive and further inoculated into the allantoic sac of the SPF eggs. The virus propagated and were characterized further using Haemagglutination (HA) test after five passages. Subsequently, viral RNA was extracted using Trizol and reverse transcribed. The 200 bp amplicon of EIV’s matrix (M) gene were successfully amplified. These results indicated that SPF eggs with one-step RT-PCR could be used for EIV replication and diagnosis of field samples. Viable quantity and quality of infectious virus particles are crucial in disease diagnosis. Poor quality and low quantity of infectious virus particles can lead to difficulties in field virus isolation and diagnosis. Even though, influenza virus in SPF eggs is not applicable for rapid diagnosis nonetheless it allowed for the recovery and propagation of the viable virus.

Keywords: Equine Influenza Virus (EIV), reverse transcriptase polymerase chain reaction (RT-PCR), specific pathogen free (SPF) eggs, virus isolation.    

Categories: 2018
Tags: Jurnal/Prosiding/Abstrak
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