Molecular characterization and phylogenetic analysis of Infectious Bursal Disease Virus from chicken in Malaysia

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  • October 22, 2021
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S. M. Redzwan. Molecular characterization and phylogenetic analysis of Infectious Bursal Disease Virus from chicken in Malaysia. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of e-Poster Presentation).

S.M.Redzwan*, A. S. Syamsiah,  B. L. Leow, M.Y.Faizu Fikri, G.H. Ong, B.Azura, Z. Maizatul,  M. R. Jihan  Veterinary Research Institute, Department of Veterinary Services, 59, Jalan Sultan Azlan Shah, 31400 Ipoh, Perak, Malaysia   Corresponding author:   

Infectious Bursal Disease Virus (IBDV) is a highly contagious disease and can cause immunosuppression in chicken. Serotype I IBDV is continuously reported and can cause significant economic losses in Malaysia despite the implementation of the intensive vaccination program. In this study, six Malaysian IBDVs in 2018 were isolated and identified using Reverse Transcription Polymerase Chain Reaction (RT-PCR) based on the VP2 gene (hypervariable region). Phylogenetic analysis revealed that two isolates in this study were of very virulent IBDV (vvIBDV) strain and four isolates were of attenuated vaccine strains. In addition, amino acids A222, I256, I294 and I299 of VP2 gene are conserved among the previously characterized very virulent IBDV strains are also encoded by those two isolates. The two vvIBDV isolates were similar and clustered together with Malaysian and Japanese strains. Therefore, the present study has demonstrated the continuous circulation of vvIBDV strains in Malaysia. Hence, VP2 gene genetic characterization of IBDV strains is important for efficient diagnosis and disease control in poultry industries.  

Keywords: Very Virulent Infectious Bursal Disease Virus (vvIBDV), Molecular characterization, Phylogenetic analysis, VP2 gene. 

Categories: 2018
Tags: Jurnal/Prosiding/Abstrak