Molecular Detection of Infectious Bronchitis Virus (IBV) from Avian Samples in RVLBT Using RT-PCR and Nested PCR

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  • October 22, 2021
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Sarenasulastri Ag. Besar. Molecular Detection of Infectious Bronchitis Virus (IBV) from Avian Samples in RVLBT Using RT-PCR and Nested PCR. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of Oral Presentation).

 

Thenamutha Muniandy, Sarenasulastri Ag. Besar 

Jabatan Perkhidmatan Veterinar  

Corresponding author: thenamutha@gmail.com 

This paper reports the detection of Infectious Bronchitis Virus (IBV) from avian samples submitted to Regional Veterinary Laboratory at Bukit Tengah (RVLBT) by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and Nested Polymerase Chain Reaction (PCR) techniques. The cases were diagnosed with established RT-PCR and Nested PCR methods as a rapid diagnostic tool for IBV. These viruses that been detected in species of birds and chicken probably have similar genome organization and in gene sequence to IBV. Nowadays, molecular detection has been widely used for detection of IBV whereby there are universal oligonucleotides pairs for detection of IBV in RT-PCR has been designed such as UTR1 & UTR2 and S1. Meanwhile, universal oligonucleotides pair UTR3 & UTR4 were used in Nested PCR to detect for greater specificity and sensitivity of IBV. Different species of avian samples were selected from cases received by RVLBT and the RNA was extracted according to the standard protocols. Overall, the avian non-poultry samples were tested positive coronavirus using oligonucleotides pair UTR3 & UTR4 while tested negative using oligonucleotide pair S1 as well as it showed significances in terms of specificity and sensitivity. Interestingly, 12/30 of the avian poultry samples were tested negative with oligonucleotides pair UTR1 & UTR2 but were then tested positive IBV with oligonucleotides pair UTR3 & UTR4. However in Malaysia, the issue of detection of positive IBV in avian samples was still insufficient even with less study was done on molecular detection of IBV from avian field samples

Categories: 2018
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