Rapid and simple approach identification of Pasteurella multocida serogroup A, B dan D by Direct PCR from colony

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  • October 22, 2021
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Mohamad Azlan Jahaya. Rapid and simple approach identification of Pasteurella multocida serogroup A, B dan D by Direct PCR from colony. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of e-Poster Presentation).

 

Mohamad Azlan Jahaya, Noor Asyikin Abu, Tan Teck Leon, Norhidayu Abd Halim  Department of Veterinary Services  Corresponding author: azlanjahaya@dvs.gov.my 

Pasteurella multocida and its serogroups is an important zoonotic pathogen which can have a considerable impact in livestock as well as damaging economic impact. The infection of this bacteria can cause a range of diseases to wild and domesticated animal as well as human. This study aimed to identify the Pasteurella multocida species and its serogroup A, B and D by conventional biochemical test and direct polymerase chain reaction (PCR) from pure colony. The results for conventional biochemical test were compared with PCR technique. The result showed that all the P. multocida genus and its serogroup A, B and D were identified by both technique and give the same results as comparison. Only a single serogroup were detected by PCR for each isolate proves that the primers were specific. This study report that PCR directly from colony is specific and comparable to conventional biochemical test with sugar fermentation test. Identification of P. multocida will become more efficient, fast and convenient due to direct PCR from colony.    

Categories: 2018
Tags: Jurnal/Prosiding/Abstrak
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