Usage of bioreactor in producing Salmonella antigen as a Salmonella biological control in poultry
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- October 22, 2021
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Muhammad Marwan Ibrahim. Usage of bioreactor in producing Salmonella antigen as a Salmonella biological control in poultry. 30th Veterinary Association of Malaysia (VAM) Congress. 19th-20th October, 2018. Hilton Hotel, Petaling Jaya, Malaysia.(Abstract of e-Poster Presentation).
Muhammad Marwan Ibrahim, Dahlia Harun, Zarrahimah Zakaria, Harnita Embi, Nur Liyana, Muhammad Razak. Department of Veterinary Services. Corresponding author: email@example.com
One of the effective methods controlling Salmonella in poultry is by regularly monitoring farm with salmonella antigen. As a step towards biomass production, this antigen has been optimized in bioreactor as an alternative from the conventional method that currently used in Veterinary Research Institute, Malaysia. This study aimed to optimize the growth of Salmonella enterica serovar Pullorum and Salmonella enterica serovar Enteritidis by utilizing bioreactor with commercialized media. It ishypothesized bacteria cultivation can be produced in a large scale, higher yield with lower risk of contamination in a bioreactor. Five strains are involved in this optimization process including four strains of S. Pullorum isolates (SP 9-25, SP 14/11, SP 690/79 and SP 7107/07) and one S. Enteritidis isolate (SE 8543/96). Each strains were successfully cultured in 3.6L bioreactor without contamination.Isolates were cultured in nutrient broth and incubated aerobically where aeration was supplied with sparger and rotor. The temperature was controlled using inlet water supply, while acidity and alkalinity were controlled using 2M hydrochloric acid and 2M sodium hydroxide respectively. The culture was monitored based on the enumeration using spectrophotometer at 600nm every two hours and each strain was harvested at optimum time. The growth of bacteria also was determined using spread plate technique before harvesting. High productivity without contamination makes the bioreactor a promising tool for the cultivation and study of salmonella antigen for industrial application.
Keywords: S. Pullorum, S. Enteritidis, Salmonella antigen, bioreactor.